内部細胞塊inner cell mass (embryoblast)と栄養膜trophoblastへの分化の分子メカニズム

Cdx2とOct4の局在

Figure 5

https://www.nature.com/articles/cr200979

Expression and localization of Cdx2 and Oct4 in Brg1 KD blastocysts. (A) ICC analysis of Oct4 and Cdx2 in Brg1 KD and control blastocysts. In control blastocysts (a-d) Oct4 expression (green) is restricted to the ICM and is largely absent in the Cdx2-positive (red) trophectoderm. In contrast, in Brg1 KD blastocysts (e-h) Oct4 is widely expressed in both the ICM and cdx2-positive (yellow) trophectoderm. Arrowheads denote coexpression of Oct4 and Cdx2. Nuclei were counter stained with DAPI (blue). (B) Quantification of the average number of cells in Brg1 KD and control blastocysts expressing Oct4, Cdx2, and Oct4 & Cdx2 (double expression). Asterisks denote statistical significance (p,0.05) between Brg1 KD and control blastocysts. A total of 25 Brg1 KD blastocysts and 15 control blastocysts were analyzed. doi:10.1371/journal.pone.0010622.g002

BRG1 Is Required for CDX2-Mediated Repression of oct4 Expression in Mouse Blastocysts https://www.researchgate.net/figure/Expression-and-localization-of-Cdx2-and-Oct4-in-Brg1-KD-blastocysts-A-ICC-analysis-of_fig7_44614272

  1. https://www.researchgate.net/figure/Expression-of-trophectodermal-and-lineage-specification-markers-in-Ncad-ki-mutants_fig2_6659600
  2. https://www.researchgate.net/figure/Specific-lineage-markers-of-ICM-Oct4-Sox2-and-TE-Cdx2-were-characterized-in-both_fig2_321294065

YAPはDNAと直接結合せず転写因子のcoactivatorとして働きます。

As YAP and TAZ are transcriptional co-activators, they do not have DNA-binding domains. Instead, when inside the nucleus, they regulate gene expression through TEAD1-4 which are sequence-specific transcription factors that mediate the main transcriptional output of the Hippo pathway.The YAP/TAZ and TEAD interaction competitively inhibits and actively dissociates the TEAD/VGLL4 interaction which functions as a transcriptional repressor.

https://en.wikipedia.org/wiki/YAP1

 

参考

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7691851/

https://www.sciencedirect.com/science/article/pii/S0969212614002068

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6821267/　この模式図を見るとOct4はエンハンサーにもプロモーターにも結合するようです。