アミノ酸配列の最後に基質も表示されています。フマラーゼ(4APB)の場合は ….LDVLAMAKAEQ FUM CA 0000000000000000000 といった具合。これは目立たないので、気付きにくいですね。
3EXFというファイルの酵素の場合は、…IFAIKKTLNI K O のように表示がありました。KとOは何か酵素のアミノ酸ではないものみたいです。それと、最後ではなく途中ですが、 … WIKFKSVS MG TPP OLQVTV… のような表示もありました。MGは何でしょう。TPPはチアミンピロリン酸(TPP)だろうと思います。
Inositol (myo-inositol, see below) was first isolated by Scherer (7), and called “inosite” because of its sweet taste.(A short history of inositol lipids Robin F. Irvine Journal of Lipid Research Volume 57, Issue 11, November 2016, Pages 1987-1994)
Inositol (hexahydroxycyclohexane) was first isolated from muscle in 1850 by Scherer. He coined the name “inositol” from the Greek inos (muscel). Nine stereoisomers of inositol are theoretically possible but only seven occur naturally, the exception being epi- and allo-inositol. Because myo-inositol is regarded as the major isomer among the inositols, with regard to both distribution and fuction, particular attention has been devoted to this compound. Although the name myo-inositol is in fact a pleonasm (it is derived from another Greek word for muscle, myo), its use has become generally accepted, and in this paper, myo-inositol will be refeerred to simply as inositol. (Plant inositides and intracellular signaling Plant Physiol (1993).103:705-709. Bjorn K. Drobak)
Three major organic acids that accumulate in most fruits include malic, citric, and tartaric acid, and their final concentration in ripening fruits depends on the balance between the biosynthesis of organic acid, their degradation, and their vacuolar storage [3,4]. Organic acids are different in various kinds of fruits. For example, citric acid is the major organic acid in citrus [5], while malic acid is the predominant organic acid in apple [6], pear [7], and loquat [2].(https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316603/)
Malate, which is one of the main organic acids, has various biological activities and pharmacological effects including the promotion of ATP production, antioxidant and anti-inflammatory activity, and the ability to reverse oxidative stress (Ding et al., 2016).(https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154052/)
解糖系を逆行して回路が閉じるのはなぜ?と不思議に思います。つまり、解糖系との関係がよくわからんというわけです。それに関しては、ストライヤーの生化学に明解な説明がありました。The flow of glucose 6-phosphate depends on the need for NADPH, ribose 5-phosphate, and ATP という節です(第3版の場合は431ページ Figure 18-2, 第8版の場合は608ページ Figure 20.23)。
The highest activity was found in lactating mammary gland and adipose tissue. Lung and liver showed to have a moderately high activity. Brain, kidney, skeletal muscle, and intestinal mucosa showed to have also a significant activity although less than other tissues. https://pubmed.ncbi.nlm.nih.gov/10630623/