OSNAの利用

The OSNA (One-Step Nucleic Acid Amplification) assay is a RT-PCR-based technique for detecting CK19 mRNA. It is a quantitative, fast, and standardised assay, and it has been validated for lymph node molecular analysis in breast and CRC [11,12,13].

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10670609/

A novel technique for pathological examination, OSNA, uses the reverse transcription loop-mediated isothermal amplification method to amplify CK19 mRNA.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403438/

1. がんリンパ節転移診断システム（OSNA™法） 遺伝子増幅検出装置 RD-200/100i　SYSMEX
2. OSNA in colorectal cancer　SYSMEX
3. One Step Nucleic Acid Amplification (OSNA) – a new method for lymph node staging in colorectal carcinomas　Published: 06 September 2010 https://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-8-83
4. Molecular Lymph Node Staging with One-Step Nucleic Acid Amplification and its Prognostic Value for Patients with Colon Cancer: The First Follow-up Study　https://link.springer.com/article/10.1007/s00268-020-05949-6