カエルのオーガナイザーその他のシグナル分子の遺伝子のマウス胚での発現

カエルの発生学で分子シグナルが詳細に調べられてきましたが、それらの分子がマウス胚でも同様の働きをもつのかどうかの情報の整理が大変です。

Goosecoid

  1. Goosecoid Regulates the Neural Inducing Strength of the Mouse Node　Developmental Biology Volume 216, Issue 1, 1 December 1999, Pages 276-281
  2. Gastrulation in the mouse: the role of the homeobox gene goosecoid　M Blum, SJ Gaunt, KWY Cho, H Steinbeisser, B Blumberg, D Bittner, EM De Robertis　Cell, 1992 本文有料
  3. TGIF1 and TGIF2 regulate Nodal signaling and are required for gastrulation. 　January 2010Development 137(2):249-59 DOI:10.1242/dev.040782　https://www.researchgate.net/figure/Regulation-of-goosecoid-expression-by-Tgifs-AEmbryos-of-the-indicated-ages-and_fig6_40812258　Regulation of goosecoid expression by Tgifs. (A)Embryos of the indicated ages and genotypes were analyzed for expression of goosecoid by in situ hybridization. The brackets indicate the extra-embryonic region. Arrowheads indicate expression in the extraembryonic region. (B)A goosecoid promoter reporter construct was used to test repression by co-expressed Tgif1 (+, 20 ng/well, ++, 50 ng/well) in transfected HepG2 cells. Cells were treated with TGFb, as indicated. Results are presented as means±s.d. of triplicate transfections, normalized to a Renilla luciferase control. Activity in the presence of Tgif1 was significantly different from the control (*P<0.05, **P<0.01), as determined by Student's t-test. (C)Expression of goosecoid in 8.0 dpc embryos of the indicated genotypes was analyzed by in situ hybridization. (D)Sections through the embryos in C. All in situ images are representative of at least three embryos. Scale bars: 250mm in A,C; 100mm in D. 
    (C)Expression of goosecoid　in 8.0 dpc embryos of the indicated genotypes　was analyzed by in situ hybridization.　(D)Sections through the embryos in C.

    Cがgoosecoidの発現。nodeに局在していることがわかります。

Chordin

下の図でaがchordinのin situ hybridazation （ｍRNAの局在）です。bcはnoggin遺伝子の発現。chordinは原始線条の前側で発現しはじめて原始結節に局在したと本文で説明されていますが、図には向きなどの記載がなくわかりにくいです。Chordin遺伝子をのノックアウトしても表現型は意外なくらいマイルド（図efg）なようです。

Figure 1

The organizer factors Chordin and Noggin are required for mouse forebrain development　Nature volume 403, pages658–661 (2000)　10 February 2000 　https://www.nature.com/articles/35001072

wntシグナリング分子

https://www.semanticscholar.org/paper/Wnt-signalling-in-mouse-gastrulation-and-anterior-Arkell-Fossat/1ebceb654e1ea878ac1bd663735be63e1a14a0fb

Canonical Wnt Signaling and Its Antagonist Regulate Anterior-Posterior Axis Polarization by Guiding Cell Migration in Mouse Visceral Endoderm Developmental Cell Volume 9, Issue 5, November 2005, Pages 639-650 https://www.sciencedirect.com/science/article/pii/S1534580705003734